{"id": 1, "reference": "J BIOCHEM 4, 915-21 (1979)", "year": 1979, "PMID": 378988, "DOI": "10.1093/oxfordjournals.jbchem.a132423", "other_links": null, "method": "GdnHCl", "measure": "CD", "protein": "Tryptophan synthase alpha chain", "source": "Escherichia coli (strain K12)", "PDB_wild": "1WQ5", "length": null, "uniprot": "P0A878", "PIR_ID": null, "swissprot": null, "mutation_code": "E49M", "mutation_based": "PDB and Uniprot", "pdb_mutant": null, "pdbs_template": null, "mutated_chain": "A", "mutation_type": "Single", "mut_count": 0, "weight": null, "temperature": 298.95, "ph": 7.0, "ddg": 4.6, "dtm": null, "blosum62": -1, "pam250": 0, "pos": 0, "neg": -2, "acc": -2, "don": 0, "aro": 0, "sul": 1, "neu": -1, "sst": "Strand", "rsa": 0.0, "phi": -118.5, "psi": 113.0, "res_depth": 3.53, "ca_depth": 4.14, "relative_bfactor": 3.47, "effect": "stabilizing"}